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pe conjugated control antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pe conjugated control antibody
    Pe Conjugated Control Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+igg+isotype+control+pe/Rabbit+mAb+IgG+XP+Isotype+Control/pm41534899-309-8-12
    Average 94 stars, based on 48 article reviews
    pe conjugated control antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: LILRB4 antibodies and uses thereof
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (anti-LILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3-4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat #65-0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat #00-8222) and methanol treatment.

    Staining:

    Article Title: LILRB4 signaling in leukemia cells mediates T cell suppression and tumor infiltration
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (anti-LILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65–0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), anti-uPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (h lilrb4 )-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Article Title: Single-cell transcriptomics of allo-reactive CD4 + T cells over time reveals divergent fates during gut GVHD
    Article Snippet: .. Intracellular staining was then performed using the eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set with the following antibodies: IFNγ (XMG1.2) BV421, IL-17A (TC11-18H10.1) – BV605, rat IgG1k isotype control – BV421, rat IgG1k isotype control –BV605, rat IgG2a isotype – AF700, mouse IgG1k isotype control – APC from Biolegend; Tcf1 (C63D9) – PE, rabbit IgG isotype control – PE from Cell Signaling Technology; Tbet (4B10) – APC, Foxp3 (FJK-16S) – AF700 from eBioscience. .. Samples were acquired on a LSRII Fortessa Analyser (BD Biosciences) and data analysed using FlowJo software (Treestar).

    Article Title: LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration.
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (antiLILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65– 0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), antiuPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. Virus construction and infection For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (hlilrb4)-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Control:

    Article Title: LILRB4 signaling in leukemia cells mediates T cell suppression and tumor infiltration
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (anti-LILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65–0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), anti-uPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (h lilrb4 )-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Article Title: Single-cell transcriptomics of allo-reactive CD4 + T cells over time reveals divergent fates during gut GVHD
    Article Snippet: .. Intracellular staining was then performed using the eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set with the following antibodies: IFNγ (XMG1.2) BV421, IL-17A (TC11-18H10.1) – BV605, rat IgG1k isotype control – BV421, rat IgG1k isotype control –BV605, rat IgG2a isotype – AF700, mouse IgG1k isotype control – APC from Biolegend; Tcf1 (C63D9) – PE, rabbit IgG isotype control – PE from Cell Signaling Technology; Tbet (4B10) – APC, Foxp3 (FJK-16S) – AF700 from eBioscience. .. Samples were acquired on a LSRII Fortessa Analyser (BD Biosciences) and data analysed using FlowJo software (Treestar).

    Article Title: The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice
    Article Snippet: Antibody , Rabbit IgG Isotype Control AF647 (Rabbit monoclonal) , Cell Signaling Technology , Cat#:3452; RRID: AB_10695811 , FC (concentration matched antibodies of interest). .. Antibody , Rabbit IgG Isotype Control PE (Rabbit monoclonal, DA1E) , Cell Signaling Technology , Cat:5742; RRID: AB_10694219 , FC (concentration matched antibodies of interest). .. Antibody , Rabbit IgG Monoclonal Isotype Control (Rabbit monoclonal, EPR25A) , Abcam , Cat#:ab172730; RRID: AB_2687931 , FC (concentration matched antibodies of interest).

    Article Title: LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration.
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (antiLILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65– 0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), antiuPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. Virus construction and infection For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (hlilrb4)-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Flow Cytometry:

    Article Title: LILRB4 signaling in leukemia cells mediates T cell suppression and tumor infiltration
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (anti-LILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65–0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), anti-uPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (h lilrb4 )-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Article Title: LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration.
    Article Snippet: Briefly, human primary AML cells were stained for the surface expression of LILRB4 (antiLILRB4-Alexa Fluor 647, Biolegend, ZM4.1, 1:100) and CD33 (anti-human CD33-FITC, Biolegend, HIM3–4, 1:100) and fixable cell viability dye eFluor 450 (Bioscience, Cat#65– 0863-14, 1:100) followed by fixation (IC fixation buffer, eBioscience, Cat#00–8222) and methanol treatment. .. After that, cells were stained for intracellular antigens by anti-p-SHP-2 (Y580)-PE (Cell signaling, Cat#13328S, 1:100), anti-pIKKα/β (S176/180) (16A6) (Cell signaling, Cat#2697, 1:100), anti-NFκB (S529)-PE (eBioscience, B33B4WP, 1:100), antiuPAR-PE (Biolegend, VIM5, 1:100), anti-Arginase-1 (D4E3M) (Cell signaling, Cat#93668, 1:100), rabbit IgG Isotype control-PE (Cell signaling, Cat#5742, 1:100), mouse IgG Isotype control-PE (eBioscience, m2a-15F8, 1:100) and anti-rabbit IgG-PE (Jackson Immunoresearch Lab, Cat#111–116-144, 1:400) for flow cytometry analysis. .. Virus construction and infection For retrovirus packaging, plasmid constructs XZ201-IRES-GFP and XZ201-human lilrb4 (hlilrb4)-IRES-GFP were mixed with PCL-ECO (2:1), followed by transfection into 293T cells using Lipofectamine 2000 (Invitrogen).

    Concentration Assay:

    Article Title: The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice
    Article Snippet: Antibody , Rabbit IgG Isotype Control AF647 (Rabbit monoclonal) , Cell Signaling Technology , Cat#:3452; RRID: AB_10695811 , FC (concentration matched antibodies of interest). .. Antibody , Rabbit IgG Isotype Control PE (Rabbit monoclonal, DA1E) , Cell Signaling Technology , Cat:5742; RRID: AB_10694219 , FC (concentration matched antibodies of interest). .. Antibody , Rabbit IgG Monoclonal Isotype Control (Rabbit monoclonal, EPR25A) , Abcam , Cat#:ab172730; RRID: AB_2687931 , FC (concentration matched antibodies of interest).



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